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Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Jumarie C, Malo C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991. PubMed: 1939345 Caco-2 cells express retinoic acid binding protein I and retinol binding protein II.

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